Dynamic changes to the skeletal muscle proteome and ubiquitinome induced by the E3 ligase, ASB2β

CA Goodman; JR Davey; A Hagg; BL Parker; P Gregorevic

Journal article

Dynamic changes to the skeletal muscle proteome and ubiquitinome induced by the E3 ligase, ASB2β

CA Goodman, JR Davey, A Hagg, BL Parker, P Gregorevic

Molecular and Cellular Proteomics | ELSEVIER | Published : 2021

DOI: 10.1016/j.mcpro.2021.100050

Download PDF

Abstract

Ubiquitination is a posttranslational protein modification that has been shown to have a range of effects, including regulation of protein function, interaction, localization, and degradation. We have previously shown that the musclespecific ubiquitin E3 ligase, ASB2β, is downregulated in models of muscle growth and that overexpression ASB2β is sufficient to induce muscle atrophy. To gain insight into the effects of increased ASB2β expression on skeletal muscle mass and function, we used liquid chromatography coupled to tandem mass spectrometry to investigate ASB2β-mediated changes to the skeletal muscle proteome and ubiquitinome, via a parallel analysis of remnant diGly-modified peptides. T..

View full abstract

University of Melbourne Researchers

Craig Goodman Author

Ben Parker Author

Paul Gregorevic Author

Related Projects (3)

INVESTIGATING FOLLISTATIN-BASED INTERVENTIONS FOR LONG TERM-PROTECTION AGAINST FRAILTY ASSOCIATED WITH CHRONIC ILLNESS AND AGING

Effective therapies are urgently needed to combat frailty arising from muscle wasting associated with chronic illness and aging. The propose..

USING GENE DELIVERY TECHNOLOGIES TO DEFINE NOVEL MECHANISMS OF SKELETAL MUSCLE ADAPTATION, AND DEVELOP MUSCLE-DIRECTED INTERVENTIONS FOR FRAILTY AND SERIOUS ILLNESS

The focus of my research is to investigate the cellular mechanisms underlying regulation of skeletal muscle size and function in health and ..

DEVELOPING A NOVEL CLASS OF THERAPEUTICS FOR MUSCLE WASTING AND FRAILTY

Effective therapeutics are urgently required to combat skeletal muscle seating associated with chronic disease and aging. This project will ..

Grants

Awarded by University of Melbourne

Funding Acknowledgements

Studies were supported by project grants (1121500, 1156562) from the National Health and Medical Research Council of Australia (NHMRC), an Early Career Fellowship (1072129, NHMRC, Australia) and Driving Research Momentum scheme (The University of Melbourne) awarded to B. L. P., and a Senior Research Fellowship (1117835, NHMRC, Australia) awarded to P. G. A. H. was supported by an Australian Post Graduate Award (Department of Education and Training, Australian Government) and a PhD stipend top-up award from the Baker Heart and Diabetes Institute Bright Sparks program.